![]() ![]() D Intensity-dependent curve of sound-induced action potentials recorded from control, dnah5 1, and dnah5 rescue larvae. A– C Representative recordings showing the action potential firing responses in lch1 neurons of control ( A), dnah5 1-null mutant ( B), and dnah5 rescue ( C) to 80 dB SPL sound (500-Hz pure tone). The sound stimulus is a 500-Hz pure tone.ĭNAH5 regulates electrophysiological responses of Cho neurons to sound stimuli. One-way analysis of variance followed by Holm–Sidak post hoc analysis was used for comparison among multiple groups at each sound intensity * P < 0.05, ** P < 0.01, NS, not significant vs Controls. Compared to wild-type larvae, the behavior scores of dnah5 rescue larvae showed no significant differences at each intensity. The score is the sum of 10 tests for each larva. E Summary of startle responses to increasing sound intensity. Right: larvae show a startle responses to sound (head contraction and turning indicated by gray arrows). Gray arrows denote the crawling direction. Left: larvae crawl freely without sound stimuli. D Schematic of the behavioral assay in wild-type larvae. Two-tailed unpaired Student’s t test was used to test the difference between Ctrl and dnah5 1 larvae. B, C Travelling distance and average speed (in 90 s) are comparable in Ctrl and dnah5 1-null mutant larvae. Gray arrows represent the travelling distance. A Crawling trajectories of Ctrl ( w 1118) and dnah5 1-null mutant larvae. All together, our findings support a critical role of DNAH5 in tuning the frequency selectivity and the sound sensitivity of larval auditory neurons.Ĭhordotonal neuron Cilia Drosophila larvae Dynein Sound sensation.ĭNAH5 is required for the sound-induced startle response in Drosophila larvae. Intriguingly, DNAH5 mutant larvae displayed an altered frequency tuning curve of the auditory organs. Furthermore, disrupting DNAH5 resulted in a decrease of spike firing responses to low-level sound in chordotonal neurons. Calcium imaging confirmed that DNAH5 functioned autonomously in chordotonal neurons for larval sound sensation. While DNAH5 mutation did not affect the cilium morphology or the trafficking of Inactive, a candidate auditory transduction channel, larvae with DNAH5 mutation had reduced startle responses to sound at low and medium intensities. Using genetic labeling, we found one of the Drosophila axonemal dynein heavy chains, CG9492 (DNAH5), was specifically expressed in larval chordotonal neurons and showed a distribution restricted to proximal cilia. However, little is known about how they respond to sound with high sensitivity. Chordotonal neurons are responsible for sound sensation in Drosophila. ![]()
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